🧬 Multi-Locus Forensic Parameter Calculator & Paternity Testing System
Professional Microhaplotype Data Analysis and Paternity Testing Platform
🧮 Online Calculation
Online Calculation
Our database provides forensic parameter calculation services, including five parameters: PD, PE, Ae, He, and HIC. The input file is the frq.hap file obtained after processing with the Plink tool. The calculation results can be downloaded online. We only support .frq.hap format files as input data.
Our database provides forensic parameter calculation services, including five parameters: PD, PE, Ae, He, and HIC. The input file is the frq.hap file obtained after processing with the Plink tool. The calculation results can be downloaded online. We only support .frq.hap format files as input data.
📊 Input Haplotype Frequency Data
Drag file here or click to select
Supported formats: .frq.hap
Supported formats: .frq.hap
📈 Calculation Results
Please enter frequency data and click calculate
📐 Forensic Parameter Calculation Formulas
1. Probability of Match (PM): PM = Σpᵢ²
pᵢ is the frequency of the i-th haplotype, representing the probability of random matching between two unrelated individuals
2. Power of Discrimination (PD): PD = 1 - 2×(Σpᵢ²)² + Σpᵢ⁴
More precise discrimination power calculation formula, considering the influence of high-order statistical terms, PD > 0.95 is excellent
3. Effective Number of Alleles (Ae): Ae = 1/Σpᵢ²
Reflects the polymorphism level of genetic markers, higher values indicate richer genetic diversity
4. Expected Heterozygosity (He): He = N/(N-1) × (1 - Σpᵢ²)
N is the number of haplotype types, expected heterozygosity calculation formula with sample correction coefficient, providing unbiased estimation
5. Polymorphism Information Content (PIC): PIC = 1 - Σpᵢ² - (Σpᵢ²)² +
Σpᵢ⁴
More precise polymorphism information content calculation, including high-order correction terms, more accurately measures the information content of genetic markers
6. Power of Exclusion-Trio (PE-Trio): PE = Σᵢ₌₁ⁿ Pᵢ(1-Pᵢ)² + Σᵢ<ⱼⁿ Pᵢ²Pⱼ²(3Pᵢ+3Pⱼ-4)
More precise paternity exclusion rate calculation, considering all possible allele combinations and their exclusion probabilities
7. Shannon Diversity Index: H = -Σ(pᵢ × log₂(pᵢ))
Entropy concept from information theory, measuring the information content and diversity of genetic markers
8. Typical Paternity Index (TPI): TPI = 1/(2×PM)
Measures the strength of evidence supporting paternity, higher values indicate stronger evidence for paternity
9. Combined Parameters:
• CPD = 1 - Π(PMᵢ) • CAe = Π(Aeᵢ) • CPE = 1 - Π(1-PEᵢ)
• CPD = 1 - Π(PMᵢ) • CAe = Π(Aeᵢ) • CPE = 1 - Π(1-PEᵢ)
Multi-locus combined discrimination power, combined effective number of alleles, and combined power of exclusion
🔍 Microhaplotype Data Query
Database Description:
Based on the vcf file of the 1000 Genomes Project in the GRCh38 version, we used VCFtools to filter out the SNP data with missing or insertion. According to the recombination rate information, the data was divided into low recombination rate region data (RecRate < 0.1 cM/Mb) and high recombination rate region data (RecRate> 5 cM/Mb). Then, we used Plink to identify haplotype blocks from the two groups of data, and selected the microhaplotypes with a length of less than 200bp and containing less than 5 loci. The database provides the information of the microhaplotypes we screened out, including forensic parameters (PD, PE, Ae, He, HIC), position on the chromosome, average recombination rate, average mutation rate, and recombination rate group, etc., which can be queried and downloaded by users.
Based on the vcf file of the 1000 Genomes Project in the GRCh38 version, we used VCFtools to filter out the SNP data with missing or insertion. According to the recombination rate information, the data was divided into low recombination rate region data (RecRate < 0.1 cM/Mb) and high recombination rate region data (RecRate> 5 cM/Mb). Then, we used Plink to identify haplotype blocks from the two groups of data, and selected the microhaplotypes with a length of less than 200bp and containing less than 5 loci. The database provides the information of the microhaplotypes we screened out, including forensic parameters (PD, PE, Ae, He, HIC), position on the chromosome, average recombination rate, average mutation rate, and recombination rate group, etc., which can be queried and downloaded by users.
📋 Query Results
Please set query conditions and click the query button
📈 Statistical Analysis
0
Total Loci
0.000
Average PD
0.000
Average PE
0
Excellent Loci
Analysis Functions: Perform statistical analysis based on calculated data or query results, including parameter distribution, quality assessment, etc. You can use your input real data, or analyze based on default example data.
📈 Analysis Results
Please click the button above to start data analysis
👨👩👧👦 Paternity Testing Analysis
Paternity Testing Instructions: Analyze paternity relationships based on 30
high-quality microhaplotype loci. Supports trio (father-mother-child) analysis.
Data Format: Each line contains Locus Haplotype1 Haplotype2 (for heterozygotes) or Locus Haplotype (for homozygotes)
Frequency Data: System includes built-in frequency database for 30 microhaplotype loci (from 1000 Genomes Project)
Data Format: Each line contains Locus Haplotype1 Haplotype2 (for heterozygotes) or Locus Haplotype (for homozygotes)
Frequency Data: System includes built-in frequency database for 30 microhaplotype loci (from 1000 Genomes Project)
📁 Data Upload Method Selection
📊 Paternity Testing Principles:
🧬 Paternity Index (PI): PI = P(observed genotype|alleged father is true father) / P(observed genotype|random male is father)
⚖️ Combined Paternity Index (CPI): CPI = ∏PI_i
📈 Probability of Paternity (PP): PP = CPI / (CPI + 1)
✅ Decision Criteria: PP > 99.9% supports paternity, PP < 0.1% excludes paternity
🧬 Paternity Index (PI): PI = P(observed genotype|alleged father is true father) / P(observed genotype|random male is father)
⚖️ Combined Paternity Index (CPI): CPI = ∏PI_i
📈 Probability of Paternity (PP): PP = CPI / (CPI + 1)
✅ Decision Criteria: PP > 99.9% supports paternity, PP < 0.1% excludes paternity
📋 Paternity Testing Results
Please enter genotype data and click start analysis